The study combined zeolite nanoparticles of appropriate size for potential applications in wastewater treatment. Horizontal Vapor Phase Growth (HVPG) Technique was set up as the creation strategy, with a beginning material of 1 g powdered zeolite. Differed parameters incorporate baking temperatures of 1100o C and 1200o C and baking duration of 6 and 8 hours, while ramp time was held at 40 minutes. Spherical zeolite nanoparticles are prevalent in the fine-size range of 101 - 2,500 nm diameter, ultra-fine zeolite particles of ? 100 nm diameter, and coarse particles of > 2,500 nm diameter. Results from the pour-plate method show minimal microbial development when exposed to the zeolite nanoparticles in contrast with wastewater tests placed on an empty quartz tube.